Problems needing attention when taking microscopic

Problems needing attention when taking microscopic images with a digital microscope

a digital microscope is a microscope with a camera as the receiving element. A camera is installed at the real image surface of the microscope. Through this photoelectric device, the optical image is converted into an image of an electrical signal, and then it is subject to size detection, particle counting and other work. The digital microscope can be used with a computer to realize synchronous preview. Digital microscopes are generally divided into two categories. One is professional digital microscopes, which are connected with the microscope by the camera. The other is to add an imaging system outside the microscope to refit the original ordinary microscope into a digital microscope. The peripherals needed are digital cameras, digital cameras, analog cameras, as well as adapter lenses connected to them

at present, when taking microscopic images with digital cameras, most of them use digital cameras (such as Nikon SLR, G7, a650, etc.), that is, cameras with camera lenses that cannot be disassembled. In this way, we need to add an intermediate lens between the microscope and the camera lens due to the cumbersome process, so that the microscopic image can be formed on the focal plane of the camera ----- we call it a relay lens

the quality of this lens directly affects the quality of the microscopic image taken. Since there is no unified inspection standard for relay mirrors at present, and there is no consensus between users and producers, it is difficult to distinguish good from bad. Therefore, there are many manufacturers, and the relay mirrors on the market are mixed with good and bad. According to our understanding of microscope work and digital photography for many years, we have compared the pictures taken by various relay mirrors at home and abroad, and summarized several methods to identify the advantages and disadvantages of relay mirrors for your reference:

I. image size

generally speaking, as long as the following figure is mainly introduced, its various configurations, such as the LCD screen that can fill the camera, are successful, and it is a qualified relay mirror. It is only a minimum standard that the relay mirror can fill the screen, while some have to use up the optical zoom of the camera to fill the screen. The optical system of such a relay mirror is defective

II. Restoration of image color

the color restoration of the captured microscopic image is also a standard for testing the relay mirror. A qualified relay mirror requires that the color of the image taken should be consistent with that observed by the microscope

III. depth of field of image

people who can take photos know that shooting an image requires a certain depth of field (except for art photos). We all have a certain sense of hierarchy when observing microscopic images. A qualified relay lens must have a depth of field not less than the objective lens of the microscope

IV. image sharpness

this is the most important index to test the relay mirror. The so-called "sharpness" is what we usually call the resolution. The level of this index directly affects the quality of the captured microscopic image

this equipment is easy to operate. In short, the final standard for identifying a relay mirror is to ensure that the image taken with a digital camera is consistent with the image quality observed in the microscope in 40 countries and regions in the world that have implemented the deposit system. (end)